RIPK1 antibody (AA 328-458) (Biotin)

Catalog No. ABIN7167471

The Rabbit Polyclonal anti-RIPK1 antibody has been validated for ELISA. It is suitable to detect RIPK1 in samples from Human.

Quick Overview for RIPK1 antibody (AA 328-458) (Biotin) (ABIN7167471)

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK1 antibody is conjugated to Biotin

Application: ELISA

Product Details anti-RIPK1 Antibody (Biotin)

Binding Specificity: AA 328-458

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Receptor-interacting serine/threonine-protein kinase 1 protein (328-458AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for RIPK1

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Alternative Name: RIPK1

Background:

Background: Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at \'Ser-728\' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via \'Lys-63\'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Aliases: Cell death protein RIP antibody, FLJ39204 antibody, OTTHUMP00000039163 antibody, Receptor (TNFRSF) interacting serine threonine kinase 1 antibody, receptor interacting protein 1 antibody, Receptor interacting protein antibody, Receptor interacting protein kinase 1 antibody, Receptor interacting serine threonine protein kinase 1 antibody, Receptor TNFRSF interacting serine threonine kinase 1 antibody, Receptor-interacting protein 1 antibody, Receptor-interacting serine/threonine-protein kinase 1 antibody, Rinp antibody, RIP 1 antibody, RIP antibody, Rip-1 antibody, RIP1 antibody, RIPK 1 antibody, Ripk1 antibody, RIPK1_HUMAN antibody, Serine threonine protein kinase RIP antibody, Serine/threonine-protein kinase RIP antibody

UniProt: Q13546

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway