Ribosome Biogenesis Protein NOP53 (NOP53) (AA 227-405) antibody
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- Target
- Ribosome Biogenesis Protein NOP53 (NOP53)
- Binding Specificity
- AA 227-405
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Cross-Reactivity
- Human, Rat
- Purification
- >95%, Protein G purified
- Immunogen
- Recombinant Human Ribosome biogenesis protein NOP53 protein (227-405AA)
- Isotype
- IgG
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- Application Notes
- Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4 - Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- Ribosome Biogenesis Protein NOP53 (NOP53)
- Alternative Name
- NOP53
- Background
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Background: Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis (PubMed:24120868). In ribosome biogenesis, may also play a role in rRNA transcription (PubMed:27729611). Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions (PubMed:21741933, PubMed:24120868, PubMed:27829214). DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation (PubMed:24120868, PubMed:27829214). It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation (PubMed:22522597). Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway (PubMed:15355975, PubMed:16971513, PubMed:27729611). May also inhibit cell proliferation and increase apoptosis through its interaction with NF2 (PubMed:21167305). May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation (PubMed:25818168). Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex (PubMed:25956029). May also regulate cellular aerobic respiration (PubMed:24556985). In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of DDX58/RIG-1 (PubMed:27824081).
Aliases: Glioma tumor suppressor candidate region gene 2 protein antibody, GLTSCR2 antibody, GSCR2_HUMAN antibody, p60 antibody, PICT1 antibody, protein interacting with carboxyl terminus 1 antibody
- UniProt
- Q9NZM5
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