RAD51 antibody (pThr309) (HRP)

Catalog No. ABIN713120

Product Details anti-RAD51 Antibody (HRP)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Binding Specificity: pThr309

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RAD51 around the phosphorylation site of Thr309 [GE(p-T)RI]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51 (RAD51 Products)

Synonyms: BRCC5 antibody, HRAD51 antibody, HsRad51 antibody, HsT16930 antibody, MRMV2 antibody, RAD51A antibody, RECA antibody, CRAD51 antibody, wu:fb38e12 antibody, zgc:77754 antibody, RGD1563603 antibody, ATRAD51 antibody, F22D1.20 antibody, F22D1_20 antibody, RAS associated with diabetes protein 51 antibody, AV304093 antibody, Rad51a antibody, Reca antibody, brcc5 antibody, rad51 antibody, rad51a antibody, reca antibody, xrad51 antibody, RAD51 recombinase antibody, RAS associated with diabetes protein 51 antibody, RAD51 recombinase L homeolog antibody, RAD51 antibody, rad51 antibody, Rad51 antibody, rad51.L antibody

Background:

Synonyms: RAD51phospho T309, BRCA1/BRCA2 containing complex, subunit 5, BRCC 5, BRCC5, DNA repair protein RAD51 homolog 1, DNA repair protein rhp51, E coli RecA homolog, HGNC:9817, Homolog of E coli RecA, homolog of S cerevisiae RAD51, HRAD51, HsRad51, HsT16930, Rad 51, RAD51 homolog RecA homolog, E. coli S. cerevisiae, RAD51 homolog, RAD51 homolog S. cerevisiae, RAD51 S cerevisiae homolog, RAD51A, RECA, RecA homolog E. coli, RecA like protein, RecA, E. coli, homolog of, recombination protein A.

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

Gene ID: 5888

Pathways: DNA Damage Repair