GRK2 antibody (pSer29)

Catalog No. ABIN7219904

This anti-GRK2 antibody is a Rabbit Polyclonal antibody detecting GRK2 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for GRK2 antibody (pSer29) (ABIN7219904)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRK2 Antibody

Binding Specificity: pSer29

Purpose: GRK 2 (phospho Ser29) Polyclonal Antibody

Specificity: Phospho-GRK 2 (S29) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S29.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human GRK 2 Phospho-Ser29

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK 2

Background: Rabbit Anti-GRK 2 (phospho Ser29) Polyclonal Antibody,ADRBK1, BARK, BARK1, GRK2, Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2,The product of GRK2 phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. G protein-coupled receptor kinase 2 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.,Beta-adrenergic receptor kinase 1

Molecular Weight: observerd band 80kDa

Gene ID: 156

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling