alpha-SMA antibody

Catalog No. ABIN7201087

Product Details

Target: alpha-SMA

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purpose: α-SMA Monoclonal Antibody

Specificity: The antibody detects endogenous α-SMA protein.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthetic Peptide

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:100000), IF (1:100-1:200), IHC-P (1:1000-1:2000).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: alpha-SMA

Background: Mouse Anti-α-SMA Monoclonal Antibody,The protein encoded by ACTA2 (actin, alpha 2, smooth muscle, aorta) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.,Actin, aortic smooth muscle

Gene ID: 59

UniProt: P62736