BRAF antibody (pSer446)

Catalog No. ABIN7224480

This anti-BRAF antibody is a Rabbit Polyclonal antibody detecting BRAF in WB, ELISA and IHC (p). Suitable for Human and Mouse.

Quick Overview for BRAF antibody (pSer446) (ABIN7224480)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRAF Antibody

Binding Specificity: pSer446

Purpose: Raf-B (phospho Ser446) Polyclonal Antibody

Specificity: Phospho-Raf-B (S446) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S446.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Raf-B Phospho-Ser446

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: Raf-B

Background: Rabbit Anti-Raf-B (phospho Ser446) Polyclonal Antibody,BRAF, BRAF1, RAFB1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1,BRAF (B-Raf proto-oncogene, serine/threonine kinase) encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF.,serine threonine-protein kinase B-raf

Molecular Weight: observerd band 110kDa

Gene ID: 673

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy