PUMA antibody (C-Term)

Catalog No. ABIN7216706

The Rabbit Polyclonal anti-PUMA antibody (ABIN7216706) specifically detects PUMA in WB, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PUMA antibody (C-Term) (ABIN7216706)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PUMA Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-PUMA Polyclonal Antibody

Specificity: PUMA Polyclonal Antibody detects endogenous levels of PUMA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human PUMA

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IF 1:100-300,ELISA 1:5000-20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: BBC3, PUMA, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosisBBC3 encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. BCL2 binding component 3 cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, BBC3 is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.

Molecular Weight: 23kD

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity