CARD11 antibody (AA 10-90)

Catalog No. ABIN7213938

The Rabbit Polyclonal anti-CARD11 antibody (ABIN7213938) specifically detects CARD11 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for CARD11 antibody (AA 10-90) (ABIN7213938)

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CARD11 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-CARD 11 Polyclonal Antibody

Specificity: CARD 11 Polyclonal Antibody detects endogenous levels of CARD 11 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human CARD 11 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CARD11

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Alternative Name: CARD 11

Background: CARD11, CARMA1, Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1The protein encoded by CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.

Molecular Weight: 130kD

Gene ID: 84433

UniProt: Q9BXL7

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, BCR Signaling