Choline Acetyltransferase antibody (Internal Region)

Catalog No. ABIN7214194

The Rabbit Polyclonal anti-Choline Acetyltransferase antibody is suitable to detect Choline Acetyltransferase in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Choline Acetyltransferase antibody (Internal Region) (ABIN7214194)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-Choactase Polyclonal Antibody

Specificity: Choactase Polyclonal Antibody detects endogenous levels of Choactase protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Choactase

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: Choactase

Background: CHAT, Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylaseCHAT encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. CHAT product (choline O-acetyltransferase) is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in CHAT have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in CHAT are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for CHAT, and some of these variants have been shown to encode more than one isoform.

Molecular Weight: 82,70kD

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development