CDK5 antibody (pTyr15)

Catalog No. ABIN7218916

The Rabbit Polyclonal anti-CDK5 antibody is suitable to detect CDK5 in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CDK5 antibody (pTyr15) (ABIN7218916)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDK5 Antibody

Binding Specificity: pTyr15

Purpose: Rabbit Anti-Cdk5 (phospho Tyr15) Polyclonal Antibody

Specificity: Phospho-Cdk5 (Y15) Polyclonal Antibody detects endogenous levels of Cdk5 protein only when phosphorylated at Y15.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cdk5 Phospho-Tyr15

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: Cdk5

Background: CDK5, CDKN5, Cyclin-dependent kinase 5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunitCDK5 encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the cyclin dependent kinase 5 encoded by CDK5 does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.

Molecular Weight: 33kD

Gene ID: 1020

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis