p21 antibody (pThr145)

Catalog No. ABIN7213074

This Rabbit Polyclonal antibody specifically detects p21 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for p21 antibody (pThr145) (ABIN7213074)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-p21 Antibody

Binding Specificity: pThr145

Purpose: p21 (phospho Thr145) Polyclonal Antibody

Specificity: Phospho-p21 (T145) Polyclonal Antibody detects endogenous levels of p21 protein only when phosphorylated at T145.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human p21 Phospho-Thr145

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: p21

Background: Rabbit Anti-p21 (phospho Thr145) Polyclonal Antibody,CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21,CDKN1A encodes a potent cyclin-dependent kinase inhibitor. Cyclin dependent kinase inhibitor 1A binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of CDKN1A is tightly controlled by the tumor suppressor protein p53, through which cyclin dependent kinase inhibitor 1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. Cyclin dependent kinase inhibitor 1A can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. Cyclin dependent kinase inhibitor 1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack CDKN1A have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for CDKN1A.,Cyclin-dependent kinase inhibitor 1

Gene ID: 1026

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy