EIF2AK2 antibody (pThr258)

Catalog No. ABIN7223640

The Rabbit Polyclonal anti-EIF2AK2 antibody (ABIN7223640) specifically detects EIF2AK2 in WB, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for EIF2AK2 antibody (pThr258) (ABIN7223640)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF2AK2 Antibody

Binding Specificity: pThr258

Purpose: PKR (phospho Thr258) Polyclonal Antibody

Specificity: Phospho-PKR (T258) Polyclonal Antibody detects endogenous levels of PKR protein only when phosphorylated at T258.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PKR Phospho-Thr258

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: PKR

Background: Rabbit Anti-PKR (phospho Thr258) Polyclonal Antibody,EIF2AK2, PKR, PRKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein k,The protein encoded by EIF2AK2 (eukaryotic translation initiation factor 2 alpha kinase 2) is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for EIF2AK2.,Interferon-induced double-stranded RNA-activated protein kinase

Molecular Weight: observerd band 62kDa

Gene ID: 5610

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C