Factor X/ Factor X LC (AA 370-450), (Cleaved-Ala41) antibody
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- Target
- Factor X/ Factor X LC
- Binding Specificity
- AA 370-450, Cleaved-Ala41
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Purpose
- Cleaved-Factor X/ Factor X LC (A41) Polyclonal Antibody
- Specificity
- Cleaved-Factor X/ Factor X LC (A41) Polyclonal Antibody detects endogenous levels of fragment of activated Factor X/ Factor X LC protein resulting from cleavage adjacent to A41.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Immunogen
- Synthesized peptide derived from the N-terminal region of human Factor X/ Factor X LC at AA range: 370-450
- Isotype
- IgG
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
- Comment
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Primary Antibody
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Target
- Factor X/ Factor X LC
- Background
- Rabbit Anti-Cleaved-Factor X/ Factor X LC (A41) Polyclonal Antibody,F10, Coagulation factor X, Stuart factor, Stuart-Prower factor,F10 encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds, the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.,Coagulation factor X
- Molecular Weight
- observerd band 50kDa
- Gene ID
- 2159
- UniProt
- P00742
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