FEN1 antibody (AA 60-140)

Catalog No. ABIN7214884

The Rabbit Polyclonal anti-FEN1 antibody (ABIN7214884) specifically detects FEN1 in WB, IHC, IF and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FEN1 antibody (AA 60-140) (ABIN7214884)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-FEN1 Antibody

Binding Specificity: AA 60-140

Purpose: Rabbit Anti-FEN-1 Polyclonal Antibody

Specificity: FEN-1 Polyclonal Antibody detects endogenous levels of FEN-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN-1

Background: FEN1, RAD2, Flap endonuclease 1, FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1Flap endonuclease 1 encoded by FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Molecular Weight: 42kD

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA