FLT3 antibody (pTyr842)

Catalog No. ABIN7220672

The Rabbit Polyclonal anti-FLT3 antibody (ABIN7220672) specifically detects FLT3 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for FLT3 antibody (pTyr842) (ABIN7220672)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FLT3 Antibody

Binding Specificity: pTyr842

Purpose: Rabbit Anti-Flt-3 (phospho Tyr842) Polyclonal Antibody

Specificity: Phospho-Flt-3 (Y842) Polyclonal Antibody detects endogenous levels of Flt-3 protein only when phosphorylated at Y842.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Flt-3 Phospho-Tyr842

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: Flt-3

Background: FLT3, CD135, FLK2, STK1, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, FLK-2, Fms-like tyrosine kinase 3, FLT-3, Stem cell tyrosine kinase 1, STK-1, CD antigen CD135FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Molecular Weight: 170kD

Gene ID: 2322

UniProt: P36888

Pathways: RTK Signaling