FOXO1 antibody

Catalog No. ABIN7214940

This Rabbit Polyclonal antibody specifically detects FOXO1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FOXO1 antibody (ABIN7214940)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-FOXO1 Antibody

Purpose: Rabbit Anti-FoxO1A Polyclonal Antibody

Specificity: FoxO1A Polyclonal Antibody detects endogenous levels of FoxO1A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human FoxO1A around the non-phosphorylation site of S329

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FoxO1A

Background: FOXO1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcomaFOXO1 belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of FOXO1 with PAX3 has been associated with alveolar rhabdomyosarcoma.

Molecular Weight: 70kD

Gene ID: 2308

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling