FOXO3 antibody (pSer253)

Catalog No. ABIN7220646

The Rabbit Polyclonal anti-FOXO3 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect FOXO3 in samples from Human, Mouse and Rat.

Quick Overview for FOXO3 antibody (pSer253) (ABIN7220646)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO3 Antibody

Binding Specificity: pSer253

Purpose: FoxO3A (phospho Ser253) Polyclonal Antibody

Specificity: Phospho-FoxO3A (S253) Polyclonal Antibody detects endogenous levels of FoxO3A protein only when phosphorylated at S253.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human FoxO3A Phospho-Ser253

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FoxO3A

Background: Rabbit Anti-FoxO3A (phospho Ser253) Polyclonal Antibody,FOXO3, FKHRL1, FOXO3A, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1,FOXO3 belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. FOXO3 likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.,Forkhead box protein O3

Molecular Weight: observerd band 97kDa

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis