FOXO3 antibody (Forkhead Box O3) (C-Term)

Details for Product anti-FOXO3 Antibody No. ABIN185248
Antigen
  • AF6q21
  • FKHRL1
  • FKHRL1P2
  • FOXO2
  • FOXO3A
  • zgc:92176
  • wu:fb50g02
  • wu:fi33f07
  • Fkhrl1
  • Foxo3a
  • FoxO3A
  • 1110048B16Rik
  • 2010203A17Rik
  • C76856
  • Fkhr2
  • FOXO-3
  • FOXO3
  • foxo3a
  • xfoxo3
  • foxO3
  • foxO
  • forkhead box O3
  • forkhead box O3A
  • forkhead box O3 L homeolog
  • FOXO3
  • foxo3a
  • Foxo3
  • foxo3.L
  • foxo3
  • foxO3
Epitope
C-Term
65
45
26
18
17
10
10
10
10
9
9
9
8
8
8
7
7
7
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
378
232
200
14
11
8
8
7
7
6
6
4
3
3
3
1
1
1
Host
Goat
322
54
6
1
Clonality
Polyclonal
Conjugate
This FOXO3 antibody is un-conjugated
21
15
15
15
15
15
15
8
7
5
5
5
2
2
2
2
2
1
Application
ELISA, Western Blotting (WB)
273
171
153
80
75
27
18
18
13
9
8
5
4
2
1
1
Options
Purpose FOXO3A / FOXO3 / FKHRL1
Immunogen Peptide with sequence C-GAKQASSQSWVPG, from the C Terminus of the protein sequence according to NP_001446.1.
Blocking Peptide Blocking peptide for this product available: ABIN369100
Sequence GAKQASSQSW VPG
Isotype IgG
Specificity Both variants represent identical protein (NP_001446.1 and NP_963853.1).
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Plasmids, Primers & others Plasmids, Primers & others FOXO3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name FOXO3 (FOXO3 Antibody Abstract)
Background FOXO3, FOXO3A, FKHRL1, AF6q21, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, forkhead box O3A, forkhead (Drosophila) homolog, FOXO2, forkhead homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, forkhead box O3
Gene ID 2309
NCBI Accession NP_001446
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis
Application Notes

DS WB Results: Approx 70 kDa band observed in Human Heart lysates (calculated MW of 71.3 kDa according to NP_001446.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Forkhead Box O3 (FOXO3) (C-Term) antibody (ABIN185248) ABIN185248 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen ret...
Image no. 2 for anti-Forkhead Box O3 (FOXO3) (C-Term) antibody (ABIN185248) ABIN185248 staining (0.5µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein...
Image no. 3 for anti-Forkhead Box O3 (FOXO3) (C-Term) antibody (ABIN185248) ABIN185248 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) wi...
Background publications Brunet, Bonni, Zigmond, Lin, Juo, Hu, Anderson, Arden, Blenis, Greenberg: "Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor." in: Cell, Vol. 96, Issue 6, pp. 857-68, 1999 (PubMed).