Insulin Receptor antibody (pTyr1355)

Catalog No. ABIN7221828

This Rabbit Polyclonal antibody specifically detects Insulin Receptor in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Insulin Receptor antibody (pTyr1355) (ABIN7221828)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Insulin Receptor Antibody

Binding Specificity: pTyr1355

Purpose: Insulin R (phospho Tyr1355) Polyclonal Antibody

Specificity: Phospho-Insulin R (Y1355) Polyclonal Antibody detects endogenous levels of Insulin R protein only when phosphorylated at Y1355.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Insulin R Phospho-Tyr1355

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: Insulin R

Background: Rabbit Anti-Insulin R (phospho Tyr1355) Polyclonal Antibody,INSR, Insulin receptor, IR, CD antigen CD220,INSR encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.,Insulin receptor

Molecular Weight: observerd band 95kDa

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity