IQGAP1 antibody (AA 220-300)

Catalog No. ABIN7215470

The Rabbit Polyclonal anti-IQGAP1 antibody is suitable to detect IQGAP1 in samples from Human and Mouse. It has been validated for WB and IHC.

Quick Overview for IQGAP1 antibody (AA 220-300) (ABIN7215470)

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IQGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IQGAP1 Antibody

Binding Specificity: AA 220-300

Purpose: Rabbit Anti-IQGAP1 Polyclonal Antibody

Specificity: IQGAP1 Polyclonal Antibody detects endogenous levels of IQGAP1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human IQGAP1 at AA range: 220-300

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IQGAP1

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Alternative Name: IQGAP1

Background: IQGAP1, KIAA0051, Ras GTPase-activating-like protein IQGAP1, p195IQGAP1 encodes a member of the IQGAP family. Ras GTPase-activating-like protein IQGAP1 contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Molecular Weight: 190kD

Gene ID: 8826

UniProt: P46940

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2