MICA antibody (AA 220-270)

Catalog No. ABIN7226986

This Rabbit Polyclonal antibody specifically detects MICA in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MICA antibody (AA 220-270) (ABIN7226986)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MICA Antibody

Binding Specificity: AA 220-270

Purpose: Rabbit Anti-HLA Class I Polyclonal Antibody

Specificity: The antibody detects endogenous HLA Class I protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthetic peptide from human protein at AA range: 220-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: HLA Class I

Background: HLA-A, HLAAHuman major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6. There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938, 3375250)

Molecular Weight: 40kD

UniProt: P01891, P01892, P04439, P05534, P10314, P10316, P13746, P16189, P18462, P30443, P30450, P30453, P30455, P30456, P30457, P30512, Q09160

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response