POLB antibody (AA 270-350)

Catalog No. ABIN7214594

This Rabbit Polyclonal antibody specifically detects POLB in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for POLB antibody (AA 270-350) (ABIN7214594)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-POLB Antibody

Binding Specificity: AA 270-350

Purpose: Rabbit Anti-DNA pol β Polyclonal Antibody

Specificity: DNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human DNA pol beta at AA range: 270-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: DNA pol beta

Background: POLB, DNA polymerase betaDNA polymerase beta encoded by POLB is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of POLB exist, but the full-length nature of only one has been described to date.

Molecular Weight: 38kD

Gene ID: 5423

UniProt: P06746

Pathways: DNA Damage Repair