TDG antibody (AA 1-80)

Catalog No. ABIN7224638

The Rabbit Polyclonal anti-TDG antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect TDG in samples from Human, Mouse and Rat.

Quick Overview for TDG antibody (AA 1-80) (ABIN7224638)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TDG Antibody

Binding Specificity: AA 1-80

Purpose: TDG Polyclonal Antibody

Specificity: TDG Polyclonal Antibody detects endogenous levels of TDG protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human TDG at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG

Background: Rabbit Anti-TDG Polyclonal Antibody,TDG, G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase,The protein encoded by TDG (thymine DNA glycosylase) belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. TDG may have a pseudogene in the p arm of chromosome 12.,G

Molecular Weight: observerd band 46kDa

Gene ID: 6996

UniProt: Q13569

Pathways: DNA Damage Repair, Chromatin Binding