TDG antibody (AA 1-80)

Catalog No. ABIN7224638

The Rabbit Polyclonal anti-TDG antibody is suitable to detect TDG in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC.

Quick Overview for TDG antibody (AA 1-80) (ABIN7224638)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-TDG Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-TDG Polyclonal Antibody

Specificity: TDG Polyclonal Antibody detects endogenous levels of TDG protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TDG at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG

Background: TDG, G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylaseThe protein encoded by TDG (thymine DNA glycosylase) belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. TDG may have a pseudogene in the p arm of chromosome 12.

Molecular Weight: 46kD

Gene ID: 6996

UniProt: Q13569

Pathways: DNA Damage Repair, Chromatin Binding