ARAF antibody (pTyr302)

Catalog No. ABIN7221902

This Rabbit Polyclonal antibody specifically detects ARAF in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ARAF antibody (pTyr302) (ABIN7221902)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ARAF Antibody

Binding Specificity: pTyr302

Purpose: Rabbit Anti-A-Raf (phospho Tyr302) Polyclonal Antibody

Specificity: Phospho-A-Raf (Y302) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Y302.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human A-Raf Phospho-Tyr302

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: A-Raf

Background: ARAF, ARAF1, PKS, PKS2, Serine/threonine-protein kinase A-Raf, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene PksThis ARAF proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 68kD

Gene ID: 369

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C