Ubiquitin antibody
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- Target See all Ubiquitin Antibodies
- Ubiquitin
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Reactivity
- Various Species
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Ubiquitin antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin.
- Characteristics
- Ubiquitin monoclonal antibody (EX-9),Ubiquitin, mAb (EX-9) detects ubiquitin conjugates and free polyubiquitin chains.
- Purification
- Ion exchange purified.,Purified from tissue culture supernatant.
- Immunogen
- Enzymatically synthesised polyubiquitin chains.
- Clone
- EX-9
- Isotype
- IgG1
- Top Product
- Discover our top product Ubiquitin Primary Antibody
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- Application Notes
- Western Blot (a starting dilution of 1:500-1:1'000 is recommended)Do not use milk as blocking solution. Use 1 % BSA in an appropriate buffer instead.In order to obtain optimal polyubiquitin lysine linkage independent reactivity immunoblotting membranes must be autoclaved prior to blocking/probing with Ubiquitin, mAb (EX-9). Omitting this step results in decreased reactivity with a slight preference for K63-linked polyubiquitin.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Liquid. In PBS containing 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 °C.
- Storage
- -20 °C
- Storage Comment
- -20°C Stable for at least 1 year after receipt when stored at -20°C.
- Expiry Date
- 12 months
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- Target
- Ubiquitin
- Alternative Name
- Ubiquitin (Ubiquitin Products)
- Synonyms
- CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody
- Pathways
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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