Proteasome 20S Beta2 Subunit antibody

Catalog No. ABIN7211651

Product Details

Target: Proteasome 20S Beta2 Subunit

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Recognizes the β2 subunit of the 20S proteasome.

Characteristics: Proteasome subunit β type-7| Macropain chain Z,Proteasome 20S beta2 subunit monoclonal antibody (MCP165)

Purification: Protein G affinity purified.,Purified from hybridoma tissue culture supernatant.

Immunogen: Dinitrophenylated proteasomes.

Clone: MCP165

Isotype: IgG1

Application Details

Application Notes: Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.,Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations.Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue.Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28 kDa.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. In PBS, pH 7.2, containing 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality 'antiserum-grade' plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.

Target Details

Target: Proteasome 20S Beta2 Subunit