Recombinant GFP antibody (Biotin)

Catalog No. ABIN7272885

The Alpaca Monoclonal anti-GFP antibody has been validated for IF, ICC and FACS. It is suitable to detect GFP in samples from Aequorea victoria and Aequorea aequorea.

Quick Overview for Recombinant GFP antibody (Biotin) (ABIN7272885)

Target: GFP (Green Fluorescent Protein (GFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Aequorea victoria, Aequorea aequorea

Host: Alpaca

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1H1

Product Details anti-GFP Antibody (Biotin)

Expression System: E.coli

Specificity: GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus, mClover3 and Citrine

Purification: Produced in: E.coli

Immunogen: Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria

Application Details

Application Notes: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: A breakthrough in the field of biology and bioluminescence started in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants here.