Recombinant mNeonGreen antibody (DBCO)
Reactivity: Branchiostoma lanceolatum
ICC, IF
Host: Alpaca
Monoclonal
1E2
DBCO
single-domain Antibody (sdAb)
Recombinant Antibody
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- Target
- mNeonGreen
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Branchiostoma lanceolatum
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- DBCO
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Application
- Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Recognizes mNeonGreen. Does not cross-react with any GFP- or dsRed, of TagBFP derivatives.
- Purification
- Produced in: E.coli
- Immunogen
- Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor
- Clone
- 1E2
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- Application Notes
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- Buffer
- sdAbs are lyophilized from PBS pH 7.4.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- Target
- mNeonGreen
- Background
- MNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
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