Recombinant mTagBFP antibody
Reactivity: Entacmaea quadricolor
FACS, ICC, IF
Host: Alpaca
Monoclonal
1H7
unconjugated
single-domain Antibody (sdAb)
Recombinant Antibody
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- Target
- mTagBFP
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Entacmaea quadricolor
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2, mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657
- Purification
- Produced in: E.coli
- Immunogen
- Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2.
- Clone
- 1H7
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- Application Notes
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconsitute with 250 µL pure water for immidate use
- Buffer
- sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- Target
- mTagBFP
- Background
- TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2.
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