Bcl-2 antibody (N-Term)

Catalog No. ABIN7273031

This Goat Polyclonal antibody specifically detects Bcl-2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Monkey and Dog.

Quick Overview for Bcl-2 antibody (N-Term) (ABIN7273031)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat, Monkey, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Bcl-2 Antibody

Binding Specificity: N-Term

Purpose: Anti-BCL2

Sequence: MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVN

Specificity: Detects a band of approximately 25 kDa by Western blot in the following cell lysate: LS174T, Jurkat and HUH.

Cross-Reactivity (Details): Reacts against human, rat, mouse, canine and monkey proteins

Purification: Epitope affinity purified

Immunogen: Recombinant affinity purified peptide derived from within residues 143 aa to the N-terminus of human BCL2 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL2

Background:

Goat polyclonal antibody to BCL2. This protein belongs to a family that act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspases.

Apoptosis Regulator Bcl-2, BCL2 Apoptosis Regulator, B-Cell CLL/Lymphoma 2, PPP1R50, Protein Phosphatase 1 Regulatory Subunit 50 antibody.

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling