TurboRFP Fluorescent Protein antibody
Reactivity: Discosoma
WB, IEM, IF, IHC (fro), IHC (p)
Host: Goat
Polyclonal
unconjugated
1 image
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- Target
- TurboRFP Fluorescent Protein
- Reactivity
- Discosoma
- Host
- Goat
- Clonality
- Polyclonal
- Application
- Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity (Details)
- Reacts with Red Fluorescent Protein
- Purification
- Epitope affinity purified
- Immunogen
- Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
- Isotype
- IgG
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- Application Notes
- WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
- Comment
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In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
- Restrictions
- For Research Use only
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- Concentration
- 3 mg/mL
- Buffer
- PBS, 20 % glycerol and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.
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- Target
- TurboRFP Fluorescent Protein
- Background
- TurboRFP (orange fluorescent protein) is a basic (constitutively fluorescent) orange fluorescent protein, monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. It has very low acid sensitivity. TurboRFP is a ~26 kDa protein that is optimally excited at a 553 nm and has a maximum of emission at 574 nm. This bright fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
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