BEBOV GP antibody

Catalog No. ABIN7383890

Product Details anti-BEBOV GP Antibody

Target: BEBOV GP (Bundibugyo Ebola Virus Envelope Glycoprotein (BEBOV GP))

Reactivity: Ebola Virus

Host: Mouse

Clonality: Monoclonal

Conjugate: This BEBOV GP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Anti-Ebola virus EBOV(subtype Bundibugyo, strain Uganda 2007) Glycoprotein/GP-RBD Polyclonal Antibody

Purification: Protein A Affinity

Immunogen: Recombinant EBOV (subtype Bundibugyo, strain Uganda 2007) Glycoprotein / GP-RBD Protein (Fc Tag), ABIN7198904

Clone: 7

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-1:5000 ELISA 1:1000-1:2000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 0.2 μm filtered solution in PBS

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for BEBOV GP

Target: BEBOV GP (Bundibugyo Ebola Virus Envelope Glycoprotein (BEBOV GP))

Alternative Name: BEBOV Glycoprotein/GP (BEBOV GP Products)

Background: Glycoprotein,GP,The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.