His Tag antibody (FITC)

Catalog No. ABIN7447733

This Chicken Polyclonal antibody specifically detects His Tag in ELISA.

Quick Overview for His Tag antibody (FITC) (ABIN7447733)

Target: His Tag

Host: Chicken

Clonality: Polyclonal

Conjugate: This His Tag antibody is conjugated to FITC

Application: ELISA

Product Details anti-His Tag Antibody (FITC)

Purpose: anti-6xHIS Tag Antibody (Chicken) - FITC Conjugated

Characteristics: FITC-conjugated chicken anti-6A-His antibody offers bright, low-background fluorescence for rapid detection of His-tagged proteins in IF, flow, and blot assays.

Purification: Antigen Affinity Purified

Immunogen: Chickens were immunized with HHHHHH conjugated with KLH (6xHIS) Eggs were collected and the Ig fraction prepared. Antibodies were solid phase adsorbed before immunoaffinity purified using the peptide immobilized on a solid phase matrix then conjugated with fluorescein isothiocyanate (FITC). Antibody concentration was determined using an absorbance at 280 nm: 1.32 equals 1.0 mg of IgY. FITC/Protein Ratio = 3.0 - 6.0.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline (PBS), pH 7.2 with 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8C

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHIS tag

Target Type: Tag