RAF1 antibody (AA 598-648)

Catalog No. ABIN7451996

The Rabbit Polyclonal anti-RAF1 antibody is suitable to detect RAF1 in samples from Human and Mouse. It has been validated for WB and IP.

Quick Overview for RAF1 antibody (AA 598-648) (ABIN7451996)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-RAF1 Antibody

Binding Specificity: AA 598-648

Purpose: Rabbit anti-RAF1/c-RAF Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 598 and 648

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1/c-RAF

Background: Background: RAF1 is a mitogen activated kinase kinase kinase (MAPKKK). In response to extracellular signals, RAF1 phosphorylates the MEK1 and MEK2 MAP kinase kinases. MEK1 and MEK2 subsequently phosphorylate and activate the ERK1 and ERK2 MAP kinases to exert pleiotropic effects on cellular functions such as growth, division, differentiation, motility, and apoptosis. RAF1 associates with the Ras family of membrane GTPases and functions downstream of Ras activity.

Gene ID: 5894

NCBI Accession: NP_002871

UniProt: P04049

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling