ROCK1 antibody (C-Term)

Catalog No. ABIN7452208

This Rabbit Polyclonal antibody specifically detects ROCK1 in WB and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for ROCK1 antibody (C-Term) (ABIN7452208)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ROCK1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-ROCK1 Antibody, Affinity Purified

Predicted Reactivity: Rat,Rabbit

Purification: Affinity Purified

Immunogen: between AA 1300 and C-term

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:5,000 - 1:15,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: Background: Rho-associated protein kinase 1 (ROCK1), also known as Rho kinase beta, is a serine-threonine protein kinase that plays an important role in cellular apoptosis, growth, cell motility and cell contraction. ROCK1 shares an overall 65 % homology in amino acid sequence with ROCK2. The ROCK1 and ROCK2 isoforms are products of two separate genes. Both ROCK1 and ROCK2 are downstream targets of the Rho A small GTP-binding protein. As downstream effectors of RhoA, ROCK1 and ROCK2 mediate changes in the actin cytoskeleton via phosphorylation of target proteins such as the MBS subunit of MLCP, MLC, ERM proteins, protein LIM kinase and adducin.

Gene ID: 6093

NCBI Accession: NP_005397

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling