KPNA4 antibody (AA 471-521)

Catalog No. ABIN7453718

The Rabbit Polyclonal anti-KPNA4 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect KPNA4 in samples from Human.

Quick Overview for KPNA4 antibody (AA 471-521) (ABIN7453718)

Target: KPNA4 (Karyopherin (Importin) alpha 4 (KPNA4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KPNA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-KPNA4 Antibody

Binding Specificity: AA 471-521

Purpose: Rabbit anti-KPNA4 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 471 and 521

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for KPNA4

Target: KPNA4 (Karyopherin (Importin) alpha 4 (KPNA4))

Alternative Name: KPNA4

Background: Background: KPNA4 (karyopherin alpha 4), also known as importin alpha 3, is a member of the importin alpha family of proteins which includes KPNA1 (importin alpha 5), KPNA2 (importin alpha 1), KPNA3 (importin alpha 4), KPNA4 (importin alpha 3), KPNA5 (importin alpha 6), and KPNA6 (importin alpha 7). The importin alpha family of proteins plays a role in the import of proteins from the cytoplasm to the nucleus. The import of proteins from the cytoplasm to the nucleus involves docking the protein to receptors associated with the nuclear pore complex (NPC) followed by translocation through the nuclear pore. Importin alpha proteins bind the nuclear localization signal (NLS) of cargo proteins and function as adaptor proteins for the nuclear pore receptor KPNB1 (importin beta 1). KPNB1 is responsible for docking the importin-cargo complex to the cytoplasmic side of the NPC for its translocation through the NPC. KPNA3 (Importin alpha 4) and KPNA4 (importin alpha 3) have been reported to mediate the nuclear import of TNF-alpha induced nuclear import of NF-kappaB p50/p65 heterodimers. RNA interference experiments have shown that KPNA4 is important for HeLa cell proliferation and the nuclear import of the Ran guanine nucleotide exchange factor, RCC1.

Gene ID: 3840

NCBI Accession: NP_002259

UniProt: O00629

Pathways: Protein targeting to Nucleus