SF3B2 antibody (AA 825-872)

Catalog No. ABIN7453784

Product Details anti-SF3B2 Antibody

Target: SF3B2 (Splicing Factor 3b, Subunit 2, 145kDa (SF3B2))

Binding Specificity: AA 825-872

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-SF3b145/SAP145 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 825 and 872

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SF3B2

Target: SF3B2 (Splicing Factor 3b, Subunit 2, 145kDa (SF3B2))

Alternative Name: SF3b145/SAP145 (SF3B2 Products)

Synonyms: 145kDa antibody, 2610311M13Rik antibody, 2810441F20Rik antibody, B230398H18Rik antibody, SAP145 antibody, SF3b1 antibody, SF3b145 antibody, SF3b150 antibody, SF3B2 antibody, cus1 antibody, sap145 antibody, sf3b145 antibody, sf3b150 antibody, Cus1 antibody, SF3B145 antibody, splicing factor 3b, subunit 2 antibody, splicing factor 3b subunit 2 antibody, splicing factor 3B subunit 2 antibody, splicing factor 3b subunit 2 L homeolog antibody, Sf3b2 antibody, SF3B2 antibody, LOC714747 antibody, sf3b2.L antibody, sf3b2 antibody

Background: Background: SF3b145/SAP145 is a 145 kDa subunit of the splicing factor 3B (SF3B) protein complex. The SF3B complex is composed of at least 8 subunits (SF3B1 through SF3B8). SF3A along with SF3B and a 12S RNA unit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. In this complex SF3B2 interacts directly with SF3B4 and associates with the pre-mRNA upstream of the branch site at the anchoring site. SF3A and SF3B may play a role in anchoring the U2 snRNP to the pre-mRNA upstream of the intron's branch site.

Gene ID: 10992

UniProt: Q13435