FEN1 antibody (AA 250-300)

Catalog No. ABIN7454602

The Rabbit Polyclonal anti-FEN1 antibody is suitable to detect FEN1 in samples from Human. It has been validated for WB, IP and IHC (fp).

Quick Overview for FEN1 antibody (AA 250-300) (ABIN7454602)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-FEN1 Antibody

Binding Specificity: AA 250-300

Purpose: Rabbit anti-Fen1 Antibody, Affinity Purified

Predicted Reactivity: Sheep,Bovine

Purification: Affinity Purified

Immunogen: between AA 250 and 300

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. 16-18 hour incubation is recommended.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: Fen1

Background: Background: Flap endonuclease 1 (Fen1) is a member of the XPG/RAD2 endonuclease family. Fen1 functions primarily as an endonuclease that removes 5'-overhangs during base-excision DNA repair and lagging strand DNA synthesis. For these processes Fen1 interacts with PCNA and the 9-1-1 DNA repair complex. Fen1 also possesses 5' to 3' exonuclease activity as well as RNase H activity.

Gene ID: 2237

NCBI Accession: NP_004102

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA