KIT antibody (AA 925-976)

Catalog No. ABIN7454909

The Rabbit Polyclonal anti-KIT antibody (ABIN7454909) specifically detects KIT in WB, IP and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for KIT antibody (AA 925-976) (ABIN7454909)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-KIT Antibody

Binding Specificity: AA 925-976

Purpose: Rabbit anti-c-Kit Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 925 and 976

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: c-Kit

Background: Background: c-Kit is the receptor for stem cell factor (mast cell growth factor). It is a transmembrane receptor with tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of the c-Kit receptor and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K) [taken from the Universal Protein Resource (UniProt) P10721].

Gene ID: 3815

NCBI Accession: NP_000213

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity