HMGN1 antibody (AA 90-100)

Catalog No. ABIN7455239

This anti-HMGN1 antibody is a Rabbit Polyclonal antibody detecting HMGN1 in WB, IF (p), IP and IHC (fp). Suitable for Human and Mouse.

Quick Overview for HMGN1 antibody (AA 90-100) (ABIN7455239)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-HMGN1 Antibody

Binding Specificity: AA 90-100

Purpose: Rabbit anti-HMGN1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 90 and 100

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:1,000 - 1:5,000

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 10 μg/mg lysate

WB: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Alternative Name: HMGN1

Background: Background: HMGN1 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA to potentially alter the interaction between the DNA and the histone octamer. The two proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function [taken from NCBI Entrez Gene (GeneID:3150)].

Gene ID: 3150

NCBI Accession: NP_004956

UniProt: P05114

Pathways: Chromatin Binding