CD45 antibody (APC)

Catalog No. ABIN7456576

The Mouse Monoclonal anti-CD45 antibody is suitable to detect CD45 in samples from Rat. It has been validated for FACS.

Quick Overview for CD45 antibody (APC) (ABIN7456576)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: OX-1

Product Details anti-CD45 Antibody (APC)

Isotype: IgG1, kappa

Application Details

Application Notes: Check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10^6 cells in 100 μL volume].

Comment:

Matching Isotype Control: APC Mouse IgG1, κ Isotype Control (ABIN7469772)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS with 0.05 % Proclin300, 1 % BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Background: Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen, LCA, T200, Ly-5,Ptprc,CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets, isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Gene ID: 19265

UniProt: P04157

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling