STAT5B antibody (C-Term)

Catalog No. ABIN7464171

This Rabbit Polyclonal antibody specifically detects STAT5B in WB, IF, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for STAT5B antibody (C-Term) (ABIN7464171)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-STAT5B Antibody

Binding Specificity: C-Term

Specificity: IP/MS validation was supported by references (PMID:30377420)

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human STAT5B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: IP/MS, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.23 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Alternative Name: signal transducer and activator of transcription 5B

Background: Signal transducer and activator of transcription 5B , STAT5,The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq]

Molecular Weight: 90 kDa

Gene ID: 6777

UniProt: P51692

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation