Estrogen Receptor alpha antibody (AA 1-190)

Catalog No. ABIN7464705

This Mouse Monoclonal antibody specifically detects Estrogen Receptor alpha in WB, FACS, IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Estrogen Receptor alpha antibody (AA 1-190) (ABIN7464705)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F3

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 1-190

Specificity: This antibody is specific for ER alpha protein, and it does not cross react with ER beta protein.

Cross-Reactivity: Human, Rabbit

Purification: Protein G purified

Immunogen: The region encoding amino acids 1-190 expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1 - 5 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7 , DDDDK-tagged ESR1-transfected 293T

Validation: Comparison, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.56 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: estrogen receptor 1

Background: Estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1,This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Molecular Weight: 66 kDa

Gene ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly