CHFR antibody (N-Term)

Catalog No. ABIN7467770

This anti-CHFR antibody is a Rabbit Polyclonal antibody detecting CHFR in ICC and IF. Suitable for Human.

Quick Overview for CHFR antibody (N-Term) (ABIN7467770)

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-CHFR Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human CHFR. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: checkpoint with forkhead and ring finger domains

Background: Checkpoint with forkhead and ring finger domains , RNF116 , RNF196,E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Molecular Weight: 73 kDa

Gene ID: 55743

UniProt: Q96EP1