ATP6V1D antibody

Catalog No. ABIN7468015

Product Details anti-ATP6V1D Antibody

Target: ATP6V1D (ATPase, H+ Transporting, Lysosomal 34kDa, V1 Subunit D (ATP6V1D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human V-ATPase D. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , U87-MG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATP6V1D

Target: ATP6V1D (ATPase, H+ Transporting, Lysosomal 34kDa, V1 Subunit D (ATP6V1D))

Alternative Name: ATPase H+ transporting V1 subunit D (ATP6V1D Products)

Synonyms: ATP6M antibody, VATD antibody, VMA8 antibody, VHA36 antibody, 1110004P10Rik antibody, Atp6m antibody, Vma8 antibody, ATPase H+ transporting V1 subunit D antibody, ATPase, H+ transporting, lysosomal V1 subunit D antibody, H(+)-transporting V1 sector ATPase subunit D antibody, ATP6V1D antibody, Atp6v1d antibody, VMA8 antibody

Background:

Synonyms: ATPase H+ transporting V1 subunit D , ATP6M , VATD , VMA8

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq]

Molecular Weight: 28 kDa

Gene ID: 51382

UniProt: Q9Y5K8

Pathways: Transition Metal Ion Homeostasis, Proton Transport