CD45RA antibody
Quick Overview for CD45RA antibody (ABIN7470588)
Target
See all CD45RA AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- CD45RA antibody (472M)
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Immunogen
- Stimulated human leukocytes
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Isotype
- IgG2a, kappa
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Tonsil: A strong membranous CD45RA immunostaining should be seen in the majority of lymphocytes.
Product Description: CD45RA antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: CD45RA immunostaining should be absent in epithelial cells.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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Expiry Date
- 24 months
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- CD45RA
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Alternative Name
- CD45RA
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Background
- Gene Alias: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
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UniProt
- P08575
Target
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