CD45RA antibody

Catalog No. ABIN7477415

The Mouse Monoclonal anti-CD45RA antibody has been validated for IHC. It is suitable to detect CD45RA in samples from Human.

Quick Overview for CD45RA antibody (ABIN7477415)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-472M

Product Details anti-CD45RA Antibody

Immunogen: Stimulated human leukocytes

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A strong membranous CD45RA immunostaining should be seen in the majority of lymphocytes.

Negative Control: Tonsil: CD45RA immunostaining should be absent in epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein,CD45RA antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08575