CD45RA antibody

Catalog No. ABIN94136

There is 1 publication for this product available. The Mouse Monoclonal anti-CD45RA antibody is suitable to detect CD45RA in samples from Human. It has been validated for FACS, WB, IHC (p), IP and MS.

Quick Overview for CD45RA antibody (ABIN94136)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Mass Spectrometry (MS)

Clone: MEM-56

Product Details anti-CD45RA Antibody

Purpose: Anti-Hu CD45RA Purified

Cross-Reactivity (Details): Human

Characteristics: The antibody MEM-56 reacts with an extracellular epitope of CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human thymocytes and T lymphocytes.

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Flow cytometry: Recommended dilution: 0.3-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD45RA antibody (ABIN94136)

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, (1990) (PubMed).

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background: CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.,PTPRCR, T200RA

Gene ID: 35