CD45RA antibody (CD45RA) (Biotin)

Details for Product anti-CD45RA Antibody No. ABIN94137
Antigen
Reactivity
Human
305
19
10
7
5
2
1
1
Host
Mouse
337
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45RA antibody is conjugated to Biotin
47
39
22
14
7
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA), Western Blotting (WB)
317
128
83
82
75
43
41
27
6
6
4
3
1
Options
Immunogen Human thymocytes and T lymphocytes.
Clone MEM-56
Isotype IgG2b
Specificity The antibody MEM-56 reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
HLDA IV, WS Code NL 907
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD45RA (CD45RA Antibody Abstract)
Background CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:120. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD45RA (CD45RA) antibody (Biotin) (ABIN94137) Western Blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymph...