CD45RA antibody (CD45RA) (FITC)

Details for Product anti-CD45RA Antibody No. ABIN94138
Antigen
Reactivity
Human
305
19
10
7
5
2
1
1
Host
Mouse
337
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45RA antibody is conjugated to FITC
46
39
23
14
7
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA), Western Blotting (WB)
317
128
83
82
75
43
41
27
6
6
4
3
1
Options
Immunogen Human thymocytes and T lymphocytes.
Clone MEM-56
Isotype IgG2b
Specificity The antibody MEM-56 reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
HLDA IV, WS Code NL 907
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD45RA (CD45RA Antibody Abstract)
Background CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD45RA (CD45RA) antibody (FITC) (ABIN94138) Western Blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymph...
Product cited in: Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).